2015年8月19日 · Immunofluorescence analysis using Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) (Product # MN1020), shows plasma membrane and cytoskeleton localization in SH-SY5Y cells treated with retinoic acid as compared to untreated SH-SY5Y cells which is reported to be low to negative for PhosphoTau expression.

2024年2月6日 · AT8 stains neurons in the brains of transgenic mice that express human tau with a mutation linked to frontotemporal dementia. Sections through the spinal cords of non-transgenic (left) and JNPL3 (right) mice.

Perikaryal and axonal staining was also strong in the pyramidal layer of hippocampal CA3 sub-region ( Figure 8 B) and layers II/III of the entorhinal cortex ( Figure 8 C), with less in the...

1995年4月21日 · As such, monoclonal antibody AT8 is widely used. By a combination of site-directed mutagenesis of recombinant tau and in vitro phosphorylation, we show that AT8 requires tau protein to be phosphorylated at both serine 202 and threonine 205 (using the numbering of the longest human brain tau isoform.

The strong positive staining for CP13 (pSer202) and weak staining for AT8 (pSer202/pThr205) suggests that Thr205 is also likely unique to AD and related tauopathies. In addition, recent data suggest that AT8 has the highest affinity for tau phosphorylated at Ser202, Ser205, and Ser208, with the latter having the greatest effect on affinity ( 32 ).

Immunoperoxidase staining for AT8-phosphorylated tau (top), ionized calcium binding adaptor molecule 1 (Iba1; middle) and cyclo-oxygenase 2 (COX-2) immunoreactivities in saline-injected controls (Sal) and at 1-3 h after systemic LPS challenge.

The approach measures the fraction of specific and nonspecific cell staining intensity within a single sample using flow cytometry. This is achieved by quantifying both the binding signal to cells expressing the wild-type tau and cells expressing tau with …

The model shows specific and consistent formation of intraneuronal insoluble full-length 4R tau inclusions, which are positive for known markers of tau pathology (AT8, PHF-1, MC-1), and creates...

The resultant detergent-insoluble tau shows deficient phosphorylation on AT8, AT100, AT180 and PHF1 epitopes, indicating inter-domain cooperativity.

Recognizes phosphorylated paired helical filament tau. Recognises tau protein phosphorylated at both serine 202 and threonine 205. No cross-reactivity with unphoshorylated tau. No Available Comments. To make a comment you must login or register. Clinton LK, Blurton-Jones M, Myczek K, Trojanowski JQ, Laferla FM.