RNA interference (RNAi), the biological mechanism by which double-stranded RNA (dsRNA) induces gene silencing by targeting complementary mRNA for degradation, is a tremendous innovation in the universal therapeutic treatment of disease and revolutionizing the way researchers study gene function.

Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.

2021年8月15日 · The mechanism of action of small interfering RNA (siRNA) is based on post-transcriptional gene silencing. siRNA molecules are usually specific and efficient in the knockdown of disease-related genes. However, they are characterized by low cellular uptake and are susceptible to nuclease-mediated degradation.

This review aims to provide an overview of the mechanism of siRNA based on RNA interference (RNAi), highlight the advantages of siRNA in drug development, and explore potential targets for cancer therapy using siRNA therapeutics.

Two primary categories of these small RNAs— short interfering RNAs (siRNAs) and microRNAs (miRNAs)—act in both somatic and germline line-ages in a broad range of eukaryotic species to regulate endogenous genes and to defend the genome from invasive nucleic acids.

2020年6月19日 · RNA interference (RNAi) is an ancient biological mechanism used to defend against external invasion. It theoretically can silence any disease-related genes in a sequence-specific manner, making...

2024年3月20日 · Understanding the intricate pharmacology of siRNA is crucial for healthcare professionals to deliver precise therapeutic interventions in various clinical scenarios.

2024年5月30日 · Mechanism of siRNA action. The following describes the process by which natural siRNA induces gene silencing via suppression of translation. Dicer is an endoribonuclease that cleaves long dsRNA, which may originate from hairpin, complementary RNAs, or RNA-dependent RNA polymerases.

One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference). The discovery of RNAi, first in plants and Caenorhabditis elegans and later in mammalian cells …

This review provides an overview of siRNA, its mechanism of action, the barriers standing in the way of their use in therapy and ways with which we are overcoming these barriers.