2016年3月30日 · Sometimes the same transcription factor can act as a repressor or an activator under different conditions. Transcription factors have specific target genes. General transcription factors (GTF), however are ubiquitous proteins that are generally needed by the RNA polymerase to initiate and carry out transcription. TATA-binding protein and TAFs ...

2016年12月27日 · Sigma factors (sigmas) are bacterial transcription factors that bind core RNA polymerase (RNAP) and direct transcription initiation at cognate promoter sites. and another paper had the title - Specific binding of the transcription factor sigma-54 to promoter DNA. However this contradicts with my book- Principle of Genetics Snustard and Simmons:

2015年3月16日 · The following domains are found in many transcription factors, and they all recognise both strands. More correctly, they recognise basepairs and their orientation. The first 5 pages of this lecture slideshow demonstrate that the chemical groups on the side of basepairs, accessible in the major groove, allow proteins to distinguish A:T, T:A, C:G ...

2013年6月11日 · I am puzzled about whether the TF only binds to DNA in the initiation stage of transcription and will detach during transcription. (I am thinking if the TF bind to the sense strand, it will block the PoLII for transcription, thus they should be removed.) Many thanks in advance.

2017年10月28日 · If you you were interested in obtaining all genes of a given transcription factor, you could buy a license, costing around 150 USD per year, for the GeneALaCart service of the company that runs the free genecards ( https://genealacart.genecards.org), upload a list of all human genes (which you could for instance obtain from ncbi's ftp server ...

2018年4月19日 · A transcription factor is a protein that regulates the much more complex and bigger RNA polymerase. Transcription factors control the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. Reference-Berg et al., Biochemistry, 5 th ed. New York: Freeman (2002) < Fig. 1.

This paper ("Essential role of microphthalmia transcription factor for DNA replication, mitosis and genomic stability in melanoma.")analyzed (among other things) the binding sites for the microphthalmia-associated transcription factor (MITF) and their distance from the transcription start site. Figure 1 a/b of the paper is shown below.

2017年5月28日 · The easiest approach to find transcription factors for any given organism would be based on sequence homology with other transcription factors. In order for this to work your organism of interest does have to be sequences, you can check this (e.g.) on …

2014年4月18日 · There's no rule that says a transcription factor must be either a repressor or an activator. The lambda repressor (CI) is in fact a repressor and activator of transcription, depending on where it is bound and to what promoter you are referring to.

A simple example of this would be the action of epinephrine (adrenaline) on hepatocytes (liver cells), where the amino acid-based hormone uses the transcription factor CREB to express the gene coding for Glycogen Phosphorylase to engage in glycogenolysis. Here I have two questions: