2016年12月5日 · Taken together, the results suggest that phosphorylating tau at T205 protects against Aβ-induced excitotoxicity, countering the idea that all tau phosphorylation is damaging. “This is the first example of a specific tau phosphorylation that has beneficial effects in the context of Aβ toxicity,” said Lars Ittner.

2019年11月22日 · Conditioned media from mouse microglia boosted phosphorylation at tau serines 396 and Ser404 in mouse neurons, pointing to soluble factors. Blocking the IL-1 receptor on neurons, or its downstream effectors, prevented p-tau. This suggested IL-1β might be a culprit. This cytokine appears to accelerate tau phosphorylation by affecting neuronal ...

2024年2月6日 · AT8 was found to be highly specific for tau phosphorylated at serine-202 in a cell-based assay designed to quantify the degree of non-specific binding of antibodies directed against defined phosphorylation sites on tau (Li and Cho, 2020). Specificity of AT8 evaluated by flow-cytometric cell-based assay.

2005年10月10日 · Whether the accumulation of p25 and tau phosphorylation are LPS-specific or inflammation-related phenomena, therefore, remains an open question. Indeed, CDK5 is a tau kinase. p25 was first purified from microtubule fractions of bovine brain and the complex CDK5/p25 was isolated and named tau protein kinase II (TPKII) ( Ishiguro et al., 1992 ).

2023年11月3日 · The authors identified an active sequence of sTREM2, the amino acid 77-89 peptide, which upon administration into the tauopathy model was responsible for protective effects on tau phosphorylation. The authors did not report whether increased sTREM2 in the P301S mice ameliorated tau-mediated neuronal loss and/or brain atrophy.

2024年1月17日 · Though mono phosphorylated p-tau202 and p-tau205 are detected in CSF, none of these techniques has been successful in detecting the 202+205 double phosphorylation in soluble tau. The CSF concentrations for all these phosphorylated species of p-tau typically increase in AD and associate more strongly with amyloid PET deposition than with tau PET ...

2024年10月6日 · At least our results indicate that phosphorylation at microtubule-binding sites S262 and S356 causes tau to dissociate from microtubules and form droplets through liquid-liquid phase separation (Soeda et al., 2024). On the other hand, when these sites are dephosphorylated and tau binds to microtubules, aggregated tau becomes aggresome-like and ...

2010年9月16日 · Interestingly, tau needs to be phosphorylated to cause this impairment, which is, incidentally, rescued by a small compound activating the tau phosphatase PP2A (van Eersel et al., 2010). This demonstrates that targeting phosphorylation of tau is a suitable strategy in treating tauopathies (Iqbal and Grundke-Iqbal, 2008).

2018年6月16日 · Human ApoE4-expressing cells showed many results confirming previous mouse model data in regard to increased tau phosphorylation, greater soluble APP detected, and enhanced Aβ production. With these cell lines, the question of whether ApoE4 represents a loss of normal ApoE function or a gain of toxic function could be proposed.

2022年7月15日 · This interdependence of tau phosphorylation can be directed by multiple tau kinases tested in the authors’ cellular assays. To this end, they show that p38 is one of the most central tau kinases linked to site-specific interdependence, which, when targeted significantly, reduced tau hyper-phosphorylation. Ultimately this suggests that there ...