The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained.

2017年10月13日 · The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained.

Characterizing protein–protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer, and far–western blot analysis is critical to understand protein function and the biology of the cell. See all …

Here we present a simple method for examination and analysis of the direct protein-protein binding interaction between two purified proteins. In the quantitative pull-down assay, one protein (the bait protein) is immobilized on beads whereas a second protein (the prey) is kept in solution.

One common technique for measuring protein-binding affinities uses flow cytometry to analyze ligand binding to proteins presented on the surface of a cell. However, cell-binding assays require specific considerations to accurately quantify the binding affinity of a protein–protein interaction.

Choosing the right protein assay kit is crucial for accurate and reliable determination of total protein concentration in a sample. There are many factors that may influence which protein assay will be optimal for a particular set of experiments, including:

Use the protein binding assay appropriate to your needs, based on the following considerations. The ultrafiltration protein binding assay (page 127) may result in high non-specific binding, but the addition of binding inhibitors as indicated in the protocol can help to alleviate this drawback.

2020年8月6日 · We apply this framework and explain underlying fundamental concepts through experimental examples with the RNA-binding protein Puf4. Molecular associations lie at the heart of biology. Their thermodynamics provides information critical for deriving a fundamental understanding of molecular functions.

2021年4月1日 · By describing how to quantify ligand binding kinetics and interpret the data, this chapter enables investigators to evaluate the role of the temporal dimension of binding activity to their targets of interest. Therapeutic molecules exert their action by interacting with specific molecular targets.

Cyprotex provides a plasma protein binding service for a wide range of species, strains and anti-coagulants. Pharmacokinetic parameters are usually determined by analysis of drug concentrations in plasma rather than whole blood.