In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations.

The Lysotracker staining protocol enables researchers to stain lysosomes, allowing for the examination of their number, morphology, and function in various cell types. This comprehensive guide unpacks the nuances of this protocol, outlining each phase from preparation to analysis, ensuring that all the relevant aspects are thoroughly examined ...

2015年3月9日 · For researchers studying the dynamic aspects of lysosome bio genesis and function in live cells, we offer LysoSensorTM probes—fluorescent pH indicators that partition into acidic organell es. The LysoSensorTM dyes are acidotropic probes that appear to accumulate in acidic organelles as the result of protonation.

2020年12月18日 · Here, we provide simple assays to measure regulated changes of lysosomal function in living cells such as (1) cleavage of a β-Glucosidase fluorogenic substrate (Harlan et al., 2016) (β-glucosidase is the enzyme mutated in Gaucher disease, the most frequent lysosomal storage disease), (2) lysosomal Cathepsin D (using a pepstatin A peptide ...

Lysosome markers are effective tools for identifying and tracking lysosomes. While LysoTracker markers target acid contents and localize within lysosomes, CellLight fluorescent fusion proteins target to the lysosomal membrane for locating and tracking of lysosomes.

Lysosome Dyes. Lysosomes are membrane-bound organelles that act as the waste disposal system of the cell. As highly acidic (pH 4-5) organelles, lysosomes contain a variety of enzymes capable of breaking down various biomolecules including …

The LysoTracker probes (Invitrogen/Molecular Probes) are used to label lysosomes in live cells, as described in this protocol. They are more selective for acidic organelles than the classically used neutral red or acridine orange dyes.

2024年9月20日 · Flow cytometry gating strategy to measure lysosome quantity by lysotracker staining (A) and membrane integrity by AO staining (B). The fluorescence of lysotracker staining is quantified by employing a 638 nm laser with a 660/10 nm bandpass filter.

2021年12月12日 · The analysis of lysosomal dimension is important to understand how cells respond to the substrate accumulation into the lysosomal compartment. In this regard, this protocol provides a useful method for monitoring lysosomal dimension in either living …

ImageStream® Endosome/Lysosome Staining Protocol in pDC Probing for Co-localization to Endosomes & Lysosomes Samples: (2 x 106 cells per test) Single fluorescent color control samples – unstained, CD71 or CD107a FITC, etc Experimental samples – untreated, positive control treatment, experimental treatment Materials: 1.