Antibody affinity can be defined as an increase in the bond strength between the antigen and its specific antibodies. This derives from a broad population of antibody molecules, some having higher affinity and others having lower affinity.

Affinity measures the strength of interaction between an epitope and an antibody’s antigen binding site. It is defined by the same basic thermodynamic principles that govern any reversible biomolecular interaction: In other words, K A describes how much antibody-antigen complex exists at the point when equilibrium is reached.

Here we review recent advances in understanding trade-offs between different antibody properties, including affinity, specificity, stability and solubility.

This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to obtain a fingerprint reflecting both affinity and active concentration of the target protein. To illustrate the protocol, we analyze the antibody response in SARS-CoV-2 omicron-naïve samples against different SARS-CoV-2 variants of concern.

2024年12月20日 · Antibody affinity refers to the strength of the binding interaction between a single antigen epitope and the paratope (binding site) of an antibody. This interaction is a fundamental measure of how well an antibody recognizes its specific antigen target.

Affinity maturation is the process by which antibodies gain increased affinity, avidity, and anti-pathogen activity and is the result of somatic hypermutation (SHM) of immunoglobulin genes in B cells, coupled to selection for antigen binding (Figure 1).

2022年2月20日 · Affinity, also known as binding affinity, is the strength of the interaction between the antigen-binding site (paratope) on an antibody and the epitope on an antigen. Critically, it refers to the strength of the individual bond between the paratope and epitope contact residues.

2024年8月2日 · High affinity ensures robust binding to the target antigen, essential for therapeutic efficacy, while specificity guarantees that the antibody targets only the intended antigen, minimizing off-target effects and potential toxicity. However, optimizing these two parameters simultaneously is a complex challenge that often involves trade-offs.

Antibody affinity is a quantitative measure of binding strength whereas antibody avidity incorporates the affinity of multiple antigen-binding sites.

Antibody affinity refers to the strength with which the epitope binds to an individual paratope (antigen -binding site) on the antibody. High affinity antibodies bind quickly to the antigen, permit greater sensitivity in assays and maintain this bond more readily under difficult conditions.