FITC (fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with the green fluorescent dye fluorescein. The isothiocyanate moiety (-N=C=S) on FITC reacts with amino-terminal and primary amine groups on the target biomolecule to form a ...

2022年3月20日 · Annexin-V（膜联蛋白-V）是一种分子量为35-36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力结合。 用荧光素（FITC、Alexa Fluor488等）标记的Annexin-V作为探针，利用流式细胞仪或荧. 一、技术原理 细胞凋亡（apoptosis）是指细胞在一定的生理或病理条件下，受内在遗传机制控制，按照自身程序主动性、生理性的死亡过程。 细胞凋亡受凋亡相关基因调控，故又称为程序性细胞死亡（programmed cell deat…

Ready-to-use dropper vial containing 1.0 ml positive human control serum with antibody specific to DNA and/or DNP nuclear antigens. This serum demonstrates a homogeneous staining reaction on Immuno Concepts’ HEp-2 cell substrate. The chromosome region of mitotic cells demonstrates the same homogeneous staining reaction.

2020年2月26日 · FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all the essential components for performing the conjugation reaction and for purifying the FITC-protein conjugates.

Anti-nuclear antibody (ANA) testing by indirect immunofluorescence (IFT) is a fundamental laboratory method for the detection of autoantibodies in systemic autoimmune rheumatic diseases (SARD) from which clinical entities such as lupus erythematosus, mixed connective tissue disease, Sjögren’s syndrome, systemic sclerosis, polymyositis, dermatomy...

The most common method of ANA testing is indirect fluorescent assay (IFA) utilizing fluorescein isothiocyanate (FITC) as the marker on the secondary antibody. The fluorescent ANA test uses the indirect fluorescent antibody technique first described by Weller and Coons in 1954.

2022年3月17日 · First termed in 1942 by A.H. Coons, FITC is a unique fluorescein molecule having an isothiocyanate reactive group (-N=C=S), which replaced the hydrogen atom located at the base of its structure. In this article, we’ll discuss FITC labeling techniques and provide tips and tricks for maximizing yield.

FITC Staining. FITC-labeled molecules can be used as a stain in cell analysis, providing a fluorescent marker that helps in identifying and studying various cellular components and processes. Fluorescein-dextran. The labeling of dextran with fluorescein via its derivative fluorescein isothiocyanate (FITC) was first described by de Belder and ...

Fluorescein (mistakenly abbreviated by its commonly-used reactive isothiocyanate form, FITC) is currently the most commonly-used fluorescent dye for FACS analysis. FITC is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines).

FITC Labelling is a technique used in immunology, biochemistry and cell biology to attach a fluorescent dye to a biological target such as a protein or antibody. The purpose is to enable the detection and measurement of this target using a technique …