Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection.

** Optimum amount needed is determined from the protocol for Lipofectamine 3000 Transfection Reagent. For your convenience, the essential components of this protocol are now available in the Gibco ™ Liver Cancer Starter Kit.

Lipofectamine ™ Reagents Go online to view related products. ∤. Lipofectamine ™ 3000 Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells and especially designed for hard to transfect cells. Important Guidelines. ∤. Make DNA-Lipofectamine ™ 3000 complexes in serum-free medium such ...

2022年12月5日 · The single use of Lipofectamine 3000 represents a great advantage over the combinational use of Lipofectamine 3000 and P3000 by reduced toxicity. The addition of P3000 contributes to an...

Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types Cell Type: Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Lipid-based transfection is based on the electrostatic interaction between DNA-transfection reagent complexes and the cell membrane. Here, reagent P3000 plays a crucial role. After this interaction, complexes enter the cell via endocytosis, leading to the formation of an endosome.

Transfection of (HEK) cells with Lipofectamine 3000 Reagent. Plate cells so they will be 70–90% confluent at the time of transfection. Prepare 2 Eppis for all DNA construct. Eppi 1: Mix 1 volume Opti-MEM with Lipofetamine and vortex. Eppi 2: Mix 1 volume Opti-MEM with P3000 and vortex.

** Optimum amount needed is determined from the protocol for Lipofectamine 3000 Transfection Reagent. For your convenience, the essential components of this protocol are now available in the Gibco™ Lung Cancer Starter Kit. The kit includes: basal medium, FBS, Lipofectamine 3000 reagent, Opti-MEM medium, and TrypLE reagent.