A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached to each other because of strings of complementary bases in the primers.

For analyzing and comparing multiple primer sequences simultaneously. Write or paste your primer sequences to the input field (upper window). The analyzer accepts text and table format (can be copied from an Excel file, for example).

2019年4月15日 · What is a Primer dimer? Primer dimers are short, unwanted and unintended PCR products caused by the amplification of primers. Two types of primer dimers are reported commonly in PCR reactions– Self primer (Homodimer) dimer …

2023年11月27日 · In summary, primer dimers are unintended DNA fragments that can form during PCR due to various factors, including primer design and annealing conditions. Identifying primer dimers is important to ensure the accuracy of your results.

Primer dimers occur when primers are accidentally designed with inverted regions. This allows them to anneal to other primer molecules and, in effect, become linear mini-templates. A measure of the strength or spontaneity of this dimerizing is the reaction's disassociation constant (DG).

Primer dimers are short sequences formed when two primers bind to one another, instead of to the target nucleic acid. They can arise through complementary sequences at the ends of the primers or through interactions that occur at higher concentrations.

2023年7月5日 · A few common causes for primer dimer in PCR are inadequate primer design, high primer concentration, low annealing temperature, Poor quality primers, prolonged PCR cycles, complementation between primers, and complex templates.

In other words, PrimerChecker℠ was developed to provide VISUAL post primer design assessment of the thermodynamic values of primers. The application allows a comprehensive visual confirmation of a predictable primer performance in PCR runs within a …

Primer dimers can form when primers preferentially self anneal or anneal to the other primer in the reaction. If this occurs, a small product of less than 100 bp will appear on the agarose gel.

Primer dimers refer to the unintended interaction between primers that result in the formation of short amplicons with sequence unrelated to any templates. You might find these chapters and articles relevant to this topic. So-Hee Kim, ... Se-Wook Oh, in Analytica Chimica Acta, 2023.