使用RFect siRNA转染试剂将靶向相关基因CD44 的siRNA转染到原代小鼠鼻咽癌细胞中进行敲低实验。RT-qPCR结果显示，转染后实验组中CD44 的mRNA表达与对照组相比可被敲低80%左右。Western blot的结果也显示，转染后实验组中CD44 的蛋白表达与对照组相比可被敲低60%左右。

HEK293T cells were transfected with siRNA of E3 ligase candidates for 24 h, followed by co-incubation with the nsp16-Flag-expressing plasmids for 48 h, treated with MG132 (10 µM) for 16h before ...

为解决胃癌治疗难题，研究人员开展壳聚糖 - RNU11 siRNA 纳米颗粒递送系统研究 ... 实验、Transwell 侵袭 / 迁移实验、细胞划痕愈合实验、细胞凋亡实验、细胞周期分析、RT-qPCR、Western blotting 等技术检测细胞的增殖、迁移、凋亡等情况。动物实验则构建了胃癌裸 ...

北京大学肿瘤医院内蒙古医院微创介入科等研究机构的研究人员针对这一问题，开展了以 “靶向 NETO2 抑制肝癌细胞增殖、侵袭和迁移并失活 STAT3/C-MYC 通路” 为主题的研究。研究发现，NETO2 敲低能够抑制 HCC 细胞的增殖、侵袭和迁移，同时使 STAT3/C-MYC 通路失活。这一研究结果意义重大，为肝癌的治疗提供了新的潜在靶点，有望改善 HCC ...

In this study, the authors demonstrated that exosomes can serve as delivery vehicles to introduce AGO2-loaded siRNA into the cytoplasm of bacteria, and in turn down-regulated gene expression of ...

Opens in a new tab or window Share on LinkedIn. Opens in a new tab or window CHICAGO -- Novel small interfering RNA (siRNA) agent lepodisiran drastically cut levels of lipoprotein(a), or Lp(a), in ...

In a new study, researchers demonstrate that exosomes can serve as delivery vehicles to introduce AGO2-loaded siRNA into the cytoplasm of bacteria which downregulate gene expression of the mRNA ...

State Key Laboratory of Organic−Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China ...