Luciferase activity was evaluated 24 h after transfection (Promega, E2920). The RIP solution containing magnetic beads containing either mouse IgG or human anti-Ago2 antibody was added to cell lysates ...

然而，肿瘤细胞能够通过多种机制逃避 IFN-γ 介导的免疫激活，导致免疫治疗失败。 Argonaute 2（AGO2）是 Argonaute 家族的关键成员，作为微小 RNA（miRNA）传感器，可对 miRNA 库的动态变化迅速做出反应，并靶向下游 mRNA 发挥多种作用。AGO2 在肿瘤免疫治疗中的作用 ...

Argonaute 2（AGO2）是Argonaute家族的重要成员，它作为微RNA（miRNA）传感器，能对miRNA池的动态变化迅速做出反应，并靶向下游mRNA，发挥不同的作用。

癌症干细胞（CSCs）来源的外泌体 miRNA 在肿瘤转移前微环境的形成、血管生成以及肿瘤转移过程中发挥着至关重要的作用。深入研究 CSCs 来源的外泌体 miRNA 的作用机制，不仅有助于我们进一步理解癌症转移的生物学过程，还为开发针对癌症转移的新型治疗策略 ...

In a new study, researchers demonstrate that exosomes can serve as delivery vehicles to introduce AGO2-loaded siRNA into the cytoplasm of bacteria which downregulate gene expression of the mRNA ...

State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on ...

These non-coding RNAs are comprised of long non-coding RNAs (lncRNA) and small non-coding RNAs [including microRNA (miRNA)] according to their nucleotides length ... using antibody against a key ...

The mechanism behind impaired siRNA-based treatment efficacy in the presence of KRAS mutations could be related to KRAS mutations promoting cellular resistance to siRNA by modulating key components of ...

MIWI is found in RNP complexes in male germ cells 35 and colocalizes with other Argonaute/PIWI family members, AGO2 and AGO3 ... including miRNA and RNA-decay pathways. Because of the presence ...

Because YBX1 is a known RNA binding protein, is secreted by cells in exosomes and physically interacts with miR-223 during the in vitro packaging assay, it met our criteria for a potential exosomal ...