eLife1 个月
Immunoglobulin M regulates airway hyperresponsiveness independent of T helper 2 allergic ...
Once the protein bands were identified, the membrane was washed with ddH2O until 80% of the Ponceau staining is removed. The remaining 20% of the stain allowed the accurate segmentation of the ...
Frontiers1 年
Genetic ablation of Lmp2 increases the susceptibility for impaired cardiac function
Similar amounts of protein were loaded on 12.5% or 8% polyacrylamide gels and subsequently blotted onto nitrocellulose transfer membranes. Equal loading was validated via Ponceau S staining and ...
Frontiers4 年
Immunofluorescence Staining of Paraffin Sections Step by Step
Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of ...