While Smith and his colleagues’ peptide libraries demonstrated the potential of phage display, the sequences were randomly generated. Scientists did not have control over the amino acid frequencies, ...

Phage display technology involves the fusion of DNA fragments encoding exogenous proteins or small‐molecule polypeptides with phage genes encoding surface proteins, which then exist on the ...

Filamentous bacteriophage fd can display multiple copies of foreign peptides in the N-terminal region of its major coat protein pVIII, 2,700 copies of which make up the virus capsid. Here we show ...

In this issue, Gishizky and coworkers describe yet another use for phage display—a method for mapping protein–protein interactions that is faster than two-hybrid systems and that allows the ...

Scientists insert variable DNA sequences coding for their proteins of interest into plasmids that carry a phage coat protein gene, an antibiotic resistance gene, and a packaging signal. Then they ...