Laemmli’s Buffer with Pyronin Y non reducing（含非还原性焦宁Y的Laemmli缓冲液），4X Laemmli’s Buffer with Pyronin Y reducing（含还原性焦宁Y的Laemmli缓冲液），4X Laemmli’s （SDS-Sample） Buffer （Laemmli（SDS样品）缓冲液）non-reducing，4X ...

Brain homogenates were diluted in 2× Laemmli SDS sample buffer at 1:1 ratio, followed by boiling for 5 min. Samples were subjected to 7.5% SDS-PAGE and electrically blotted onto polyvinylidene ...

The supernatants were then carefully removed and saved for total protein measurements (Lowry assay) and Western blotting. Protein samples were mixed with Laemmli sample buffer (125 mM Tris–HCl, 10% ...

Whether the copy to DAC is done by interrupt or DMA, there is an intermediate storage of samples in memory that is shared between two tasks: sample generation and copy to DAC. That means it requires ...

Western blotting was performed as previously described. (44) Briefly, denatured proteins in Laemmli sample buffer were resolved in 10% SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted ...

RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote ...

Next, 90% of the supernatant were carefully removed before washing beads with 500 μL wash buffer. Then the tubes were centrifuged at 5000 g, 4°C for 3 min, and supernatant was carefully removed before ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Greatwall, a conserved MAST-like kinase with ...

In 2020-2021, a mysterious skin disease affected fishermen in Senegal. High levels of Portimine A toxin, produced by the ...

Meiosis is a type of specialized cell division through which germ cells reduce their chromosome composition from diploid to haploid. Faithful completion of the first meiosis is therefore a critical ...