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Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...
The single molecules are amplified on the bead by PCR. Finally the emulsion is broken, transforming a population of molecules to a population of beads in a roughly 1:1 manner. According to ...
EpicPCR was developed in the groups of Marko Virta and Eric Alm (MIT). The idea of epicPCR (Emulsion, Paired Isolation and Concatenation PCR) is to link functional genes and phylogenetic markers in ...