CRISPR-Cas9 is considered a revolutionary gene editing tool, but its applications are limited by a lack of methods by which it can be safely and efficiently delivered into cells. Recently, a ...

CRISPR–Cas9 has limitations. One of the most concerning, in regards to using genome editing for therapeutic applications for humans, is off-target DNA cleavage. Although some Cas9 variants have ...

The Cas9 endonuclease is guided to its targets by single guide RNAs (sgRNAs). Looking to improve the DNA targeting-specificity of Cas9, Slaymaker et al. hypothesized that DNA strand separation ...

Here, Cas9 scans for the right spot on the DNA to make an incision, then around 30 seconds in makes the cut, creating “cleaved DNA.” The next step for a successful edit would be for the cell’s ...

The predicted orientation of the Cas9 protein on the AFM substrate is shown, and a simulated AFM image calculated in the scanning view perspective shows remarkable agreement with previous HS-AFM ...

CRISPR-Cas9 tools have recently created a buzz in the global healthcare industry, with the development of numerous applications-focused solutions — and intensifying patenting disputes. The ...

A compact RNA-guided enzyme, NovaIscB, derived from bacterial IscBs—evolutionary ancestors of Cas9—has been engineered for efficient, programmable genome editing in human cells.

The research article, “ Cryo-shocked tumor cells deliver CRISPR-Cas9 for lung cancer regression by synthetic lethality, ” was published in Science Advances. Cell-based gene editing delivery ...

The University of Bayreuth's Biomaterials research group has, for the first time, successfully applied the CRISPR-Cas9 gene-editing tool to spiders. Following the genetic modification, the spiders ...