“RNA-Seq [...] is expected to revolutionize the ... Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays. Science 296, 907–910 (2002).

but little is known about the fidelity of transcriptional initiation by RNA polymerase II in vivo. I suggest that ∼90% of Pol II initiation events in yeast represent transcriptional noise ...

The N-terminal ATP-dependent ligase domain belongs to the covalent nucleotidyltransferase enzyme superfamily that includes classic RNA/DNA ligases and mRNA capping enzymes. We’ve shown that: (i) each ...

Enzymes called RNA polymerases transcribe DNA into RNA. RNA is the intermediary that carries the information contained within DNA to the ribosomes, where the genetic information is synthesized into ...

Uracil forms hydrogen bonds with adenine in RNA, contributing to the molecule's structure and function. (Image: Public Domain) Uracil was first discovered in 1900 by the Italian chemist Alberto Ascoli ...

It is compatible with cultured animal cells, tissues, blood, bacteria, yeast, fungi, and plants. Both total RNA and proteins are column-purified in under 25 minutes using a single column.