In this installment, I primarily discuss considerations to keep in mind when choosing buffering additives that will be used ...

2021). The reaction mixture consisted of potassium phosphate buffer (pH 5) + 100 μl NBT + 200 μl of Triton X + 200 μl of methionine + enzyme extracts. Approximately 100 μl were dissolved in 800 μl of ...

A high potassium level can occur if you have certain health conditions, including kidney disease or heart failure. But it can also have other causes, including certain medications and heavy ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

CD spectra were obtained using a Jasco J-1100 circular dichroism at 25°C. Sequence was resuspended in 10mM potassium phosphate pH 7.5 buffer and diluted to 12.5 µM (per strand) RNA. The CD measurement ...

After growth for 36 h, cells were harvested from LB by centrifugation (10,000 rpm, for 10 min at 5 ºC). The pellet was washed twice with phosphate buffer (50 mM, pH 7.0) and re-suspended in 10 mL of ...

This study aimed to investigate the impact of individual drought, heat, and combined drought and heat stress on twelve cotton genotypes, including eight tolerant and four susceptible genotypes. A ...

Mitochondrial DNA (mtDNA) is actively removed during spermatogenesis in a conserved process that enforces the uniparental ...

Feb. 12, 2025 — Using genes borrowed from bacteria, researchers have demonstrated fish and flies can be engineered to break down methylmercury and remove it from their bodies as a less harmful ...

was carried out on the supernatant according to the manufacturer's instructions to concentrate the complex peptide mixtures and buffer exchange the mixtures into 5% ACN, 0.5% acetic acid.