Hybridization reactions containing 10.0 μg of total cell RNA and 2.5 × 10 4 c.p.m. of antisense probe in 30 μl of hybridization buffer [80% formamide, 0.4 M NaCl, 1 mM EDTA, 40 mM PIPES (pH 6.4 ...

Two upgraded versions of GLORI have addressed the issue and enable absolute quantification of m 6 A from ultra-low RNA starting material. News & Views 07 May 2025 Nature Methods Volume: 22, P ...