Quantitative real-time technology dominates the market today but digital PCR is on the rise. A series of rodent experiments showed that even with abundant food and water, personal space is essential ...

Analyze the PCR product by agarose gel electrophoresis, and quantify the DNA yield using the PicoGreen dsDNA kit. The amount of DNA used in the emulsion PCR can vary over a relatively wide range.

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...

EpicPCR was developed in the groups of Marko Virta and Eric Alm (MIT). The idea of epicPCR (Emulsion, Paired Isolation and Concatenation PCR) is to link functional genes and phylogenetic markers in ...