There should be a dose-dependent effect on the cellular phenotype. Oligo design. In the first instance, the siRNA sequence should be carefully designed to ensure that it targets the specific ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...

Even though the delivered siRNA was designed for possessing full sequence complementarity to the bacterial mRNA, downregulation of the target protein was achieved at the translational level rather ...