There should be a dose-dependent effect on the cellular phenotype. Oligo design. In the first instance, the siRNA sequence should be carefully designed to ensure that it targets the specific ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...