The PCR cycling reactions are carried out in an automated thermocycler, which is capable of heating and cooling the reaction tubes in a short span of time. PCR-Machines are classified depending on ...

Pool the emulsified PCR reactions in a 1.7-ml microcentrifuge tube and centrifuge at 13,000g for 5 min at 25 °C. Dispose of the upper (oil) phase. Several extractions with an organic solvent will ...

1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...

Designed for uniform illumination of samples in clear microplates, larger tubes, and other transparent vessels, this device builds on the success of its predecessor and enhances capabilities for ...