Renewed efforts were made to understand the basic biology of gene transfer, the molecular biology of vectors and the cellular mechanisms ... For lentivirus HIV, the viral pre-integration complex ...

The ‘transfer plasmid’ or lentiviral vector contains the transgene of interest, while the ‘envelope plasmid’ contains genes for proteins that appear on the virus surface, the ‘packaging ...

1996b) , the vector pCMVΔR8.2 supplies all but the HIV envelope in trans, the vector pMD.G is used to produce the VSV-G pseudotype, while the transgene (lac Z) is inserted into the plasmid pHR’, which ...