called lacI, is inhibited by the ligand allolactose, to which the repressor protein directly binds. Thus, lactose, from which allolactose is formed, induces the expression of the lac operon and of ...

10xHIS—C-terminal decahistidine tag; KanR2—kanamycin A resistance gene; HS—hok/sok post—segregation killing locus; lac I—gene of the LacI repressor protein; T7 prom—promoter for ...

This specificity narrows the ability to use the LacI system in cases where researchers can’t insert this sequence, prompting a search for a more flexible repressor protein that can recognize more ...

The operon is regulated by Lac repressor, the product of the lacI gene, which is transcribed from its own promoter (PI). The repressor inhibits transcription by binding to the lac operator (O).