called lacI, is inhibited by the ligand allolactose, to which the repressor protein directly binds. Thus, lactose, from which allolactose is formed, induces the expression of the lac operon and of ...

LacI is a tetrameric protein, using two dimers to bind two specific, identical DNA sequences. This specificity narrows the ability to use the LacI system in cases where researchers can’t insert this ...

10xHIS—C-terminal decahistidine tag; KanR2—kanamycin A resistance gene; HS—hok/sok post—segregation killing locus; lac I—gene of the LacI repressor protein; T7 prom—promoter for ...