1). In the 'off' state, the Lac inhibitor represses the lac operon, which prevents expression of the Tet repressor and the target gene. Additionally, a short hairpin RNA (shRNA) against the target ...

Mutations that disrupt the trpR gene lead to elevated production ... For example, repression of the lac operon by its repressor, called lacI, is inhibited by the ligand allolactose, to which ...

They inserted these two sequences upstream of a reporter gene and used a colorimetric assay to determine whether the proteins ... create something that could create a DNA loop that was as strong as ...