Glutamate acts at NMDA receptors in cultured ... Cells were scraped, and the cell scrapings were sonicated and stored at –70°C. Protein concentration of the samples was measured by the Bradford ...

View Full Profile. Learn about our Editorial Policies. When I expressed the different protein constructs in neuron cells in culture, I demonstrated that these C-terminal tails determined the ...

For these new indicators, they swapped the type of GFP protein, producing a more photostable reporter with a brighter fluorescent signal. They also altered one amino acid in the glutamate binding ...