Fluorogenic probe to detect enzyme linked to early stage of Alzheimer’s . Indian Institute of Science (IISc) Journal Analytical Chemistry DOI 10.1021/acs.analchem.3c00099. Keywords ...

The probe combines a fluorophore and a zinc-binding region. It fluoresces only when it binds to both zinc atoms in the enzyme active site. In the presence of inhibitors that block the active site ...

The team's probe works by detecting an enzyme that is released in large quantities during IPHs, called heme oxygenase-1 (HO-1). Previous attempts to screen for HO-1 have been unreliable and cannot ...

But as the probe comes into contact with HO-1, the enzyme breaks a bond connecting these two compartments, and causes a build up of the fluorescent molecules in the donor compartment.

Now Liu has developed a probe that could cling to those enzyme-coated antibodies and make them glow under fluorescent light. The journal Analytic Chimica Acta (DOI: 10.1016/j.aca.2017.02.039) recently ...