Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...

Analyze the PCR product by agarose gel electrophoresis, and quantify the DNA yield using the PicoGreen dsDNA kit. The amount of DNA used in the emulsion PCR can vary over a relatively wide range.

One technique that researchers employ to reach these goals is high-throughput PCR that takes place in an emulsion. A water phase containing the PCR solution—primers, polymerase, nucleotides, and ...

EpicPCR was developed in the groups of Marko Virta and Eric Alm (MIT). The idea of epicPCR (Emulsion, Paired Isolation and Concatenation PCR) is to link functional genes and phylogenetic markers in ...