“This idea evolved into the notion that if you have two primers that are interacting with recombinase proteins and they move in opposite directions along a double-stranded piece of DNA, wouldn’t you ...

Since the COVID-19 pandemic, pretty much everybody is familiar with this technology: paper-based rapid test strips, also ...

A team of scientists has created a new guide to advance standards for collecting and cataloging DNA from marine species, ...

1| Add 0.001–100 ng of poly(A) + mRNA or 0.01–1 μg of total RNA into each of three microcentrifuge tubes. Adjust the volumes to 9 μl with water. Denature the RNA by heating at 75 °C for 5 ...

This is done without the need for nucleic acid amplification. In CRISPR/Cas-based diagnostic tests, guide RNAs bind pathogen DNA or RNA, triggering Cas enzymes to become active and cleave the ...

Their CRISPR-Cascade reaction utilizes two ribonucleoprotein complexes, T1 and T2, to achieve amplification-free detection of pathogenic DNA. Upon target recognition, Cas12a in the T1 complex ...