Experimental setup for the multimodal nonlinear optical microscopy of multiphoton excitation fluorescence (MPEF) and second harmonic generation (SHG) imaging. A femtosecond pulse from a tunable ...

The emergence of imaging schemes capable of overcoming Abbe's diffraction barrier is revolutionizing optical microscopy. In 1873, the German physicist Ernst Abbe realized that the resolution of ...

For decades, microscopy specimens have most often been chemically fixed because of the stability of fixed tissue and improved optical transparency of biological tissues that have been dehydrated ...