“If your cell culture substrate doesn’t have the right ... “If cells are removed from their natural home and put into a petri dish, they don't maintain their normal phenotype.

The size of the cell clumps after pipetting is critical ... representing the majority of surface area of the culture dish. Ensure an even distribution of cells across the plate.

However, one culture condition that researchers often overlook is oxygen consumption. “Cells are grown in aqueous medium and there's a cell monolayer, which generates an oxygen sink where cells are ...